Agron. Sustain. Dev.
Volume 25, Number 3, July-September 2005
Page(s) 389 - 393
Agron. Sustain. Dev. 25 (2005) 389-393
DOI: 10.1051/agro:2005034

Effect of P on nodule formation and N fixation in bean

Saber Kouas, Nahla Labidi, Ahmed Debez and Chedly Abdelly

Laboratoire d'Adaptation des Plantes aux Stress Abiotiques, INRST, BP 95, 2050, Hammam-Lif, Tunisia

(Accepted 25 April 2005)

Abstract - The present study compares the demands for P of the initial nodule formation, and of the later growth and functioning of the nodulated root system in two inoculated lines of common bean (Coco blanc and BAT477). After germination and inoculation, seedlings were divided into two lots. One lot was grown under constant P supply, either 15 (low) or 250 (high) µM P, corresponding to provision of 120 and 2000 µmol P, respectively. In the second, seedlings were cultivated on the same medium supplied with 15 µM P for 24 days after germination, and then with 60 or 250 µM (total provision of P: respectively, 390 and 1530 µmol). Nodule number and biomass were significantly diminished by the low P (120 µmol) treatment, as compared with the other treatments. However, the intrinsic characteristics of the nodules (individual biomass and size, P concentration and efficiency of N fixation) did not depend on P availability. Although the bean line BAT477 was distinguished from the Coco blanc line through higher nodule number, size, biomass, and nitrogen fixation, both lines displayed analogous responses to P availability.

Key words: Phaseolus vulgaris / P availability / nodulation / P allocation / symbiotic nitrogen fixation

Corresponding author: Chedly Abdelly

© INRA, EDP Sciences 2005