Agron. Sustain. Dev. 25 (2005) 285-291
DOI: 10.1051/agro:2005004

Microalgae community structure analysis based on 18S rDNA amplification from DNA extracted directly from soil as a potential soil bioindicator

A. Bérard^a, U. Dorigo^b, J.F. Humbert^b and F. Martin-Laurent<sup>c

a</sup>  INRA-CSE, site Agroparc, Domaine Saint Paul, 84917 Avignon Cedex 9, France
^b  INRA, Station d'Hydrobiologie Lacustre, UMR CARRTEL, BP 511, 74203 Thonon Cedex, France
^c  UMR Microbiologie et Géochimie des Sols, INRA/Université de Bourgogne, BP 86510, 21065 Dijon, France

(Accepted 28 May 2004)

Abstract - Soil algae are photosynthetically active microorganisms showing changeable community structure, depending on the soil type, the agricultural practices and the application of pesticides. To characterise algal community structure, molecular approaches complementary to classical microbiological approaches based on the isolation and the culture of soil algae are required. Our study describes a polymerase chain reaction (PCR) approach targeting algal 18S rDNA sequences of desoxyribonucleic acid (DNA) samples extracted either from unialgal eukaryotic microalgae culture, complex assemblages of microalgae populations or natural soil communities. Our first results showed that microalgae rDNA can be amplified by PCR from soil DNA samples. They also indicated difficulties extracting DNA from diatoms directly from soils, probably because of the presence of robust silicate valves. An 18S rDNA library has been established and preliminary phylogenetic analysis showed the feasibility of applying molecular methods to studying edaphic algae community structure. This is promising for soil algae ecology and for developing soil biological indicators.

Key words: algae communities / soil / amplified rDNA

Corresponding author: F. Martin-Laurent fmartin@dijon.inra.fr

© INRA, EDP Sciences 2005